Experimental autoimmune encephalomyelitis, sometimes experimental allergic encephalomyelitis (EAE) is an animal model of brain inflammation. It is an inflammatory demyelinating disease of the central nervous system (CNS). It is mostly used with rodents and is widely studied as an animal model of the human CNS demyelinating diseases, including multiple sclerosis and acute disseminated encephalomyelitis (ADEM). EAE is also the prototype for T-cell-mediated autoimmune disease in general.
EAE can be induced in a number of species, including mice, rats, guinea pigs, rabbits and primates. The most commonly used antigens in rodents are spinal cord homogenate, purified myelin, myelin protein such as MBP, PLP, and MOG, or peptides of these proteins, all resulting in distinct models with different disease characteristics regarding both immunology and pathology. It may also be induced by the passive transfer of T cells specifically reactive to these myelin antigens. Depending on the antigen used and the genetic make-up of the animal, rodents can display a monophasic bout of EAE, a relapsing-remitting form, or chronic EAE. The typical susceptible rodent will debut with clinical symptoms around two weeks after immunization and present with a relapsing-remitting disease. In order to better support the research and treatment of EAE, Pharmalegacy established EAE model by MBP, MOG or PLP induced in rats or mice.
· Female Lewis rat, 7-9 wks old.
· Guinea pig MBP (25ug for immunization)
· Complete Freud’s adjuvant (CFA)
· Immunization: footpad injection (50ul)
· FTY-720 (1mg/kg, day0-22), p.o, q.d
Aim: To examine the effect of FTY-720 on MBP-induced EAE in Lewis rats
Animal: Lewis rats, female, 170-190 g (used in experiment)
Reagent: MBP, Complete Freund’s adjuvant (CFA), Mycobacterium tuberculosis H37Ra, FTY-720