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Objective to establish the model about concanavalin A induced（Con A）experimental liver injury in mice. Methods Forty Balb/C mice were randomly divided into 4 groups (ten for each group), A, B, C and D. Con A 10mg/kg, 20mg/kg or 30mg/kg was intravenously (tail vein) injected into the mice in group A, B and C respectively while normal saline was used for the group D (control group). Activity of alanine transaminase (ALT), death rate and liver histopathological changes were observed after 8 hours of injection. The optimal Con A dose (20mg/kg) was selected, activity of ALT, death rate and liver histopathological changes were observed based on such dosage. Results after 8 hours of injection of Con A, no dead mouse was found in group A and D, but 3 and 10 mice were dead in group B and C respectively. Liver histopathology showed only degeneration of liver cells was present in group A, while degeneration, necrosis and liver inflammatory cells infiltration in group B and C. Within 24 hours of injection of 20mg/kg of Con A, the death rate, activity of ALT, liver inflammatory cells infiltration, hepatocyte degeneration and necrosis exacerbated with the prolongation of time. Conclusion the optimal dosage to establish Con A induced experimental liver injury in mice is 20mg/kg, with the optimal observation time at 8 hours after intravenous (tail vein) injected.
