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Pharmalegacy has developed HTRF technology for antibody screening, which is high-throughput, fast, easy to perform and conserves costly antigen Nanobody high-throughput screening platform screening druggable nanobodies form immunized Alpaca can yield 100+ unique positive clones with epitope binning information in shorter time than traditional phage method. High-throughput and automated equipment are involved in this platform. We also developed a new epitope binning method which can handle hundreds of antibodies in a very short time.
Ø VHH gene can be obtained by nest PCR, amplification is more efficient using our self-designed degenerate primers.
Ø OmpA leading sequence is helpful for nanobody secreting into periplasmic space, HA-tag is used for ELISA and HTRF testing.
Advantages of HTRF method
· Wash-free, fast, high throughput, compatible with automation equipment
· Significant signal, high signal-to-noise ratio
